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High Performance Liquid Chromatography of 2,5‐Dimethyl‐4‐Hydroxy‐3 (2H)‐Furanone in Pineapple and Grapefruit Juices
Author(s) -
LEE HYOUNG S.,
NAGY STEVEN
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb13996.x
Subject(s) - chemistry , high performance liquid chromatography , chromatography , flavor , methanol , extraction (chemistry) , sodium acetate , food science , cartridge , organic chemistry , mechanical engineering , engineering
A reversed‐phase HPLC method was developed for rapid analysis of 2,5‐dimethyl‐4‐hydroxy‐3(2H)‐furanone (DMHF), the compound responsible for the “burnt‐pineapple” flavor in pineapple juices. A Zorbax ODS column was used with a mobile phase of 0.05M sodium acetate (pH 4.0)/methanol (70:30); detection was by UV at 290 nm. Sample clean up was accomplished by solid‐phase extraction with C‐18 Sep‐Pak cartridges. DMHF contents ranged from 1.6 to 27.3 ppm from 10 fresh pineapple juices. Using this system, the production of DMHF was monitored in canned grapefruit juices during storage at varying temperatures.