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A Rapid Direct Extraction‐Derivatization Method for Determining Sugars in Fruit Tissue
Author(s) -
LONG A.R.,
CHISM G.W.
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb13993.x
Subject(s) - chemistry , sucrose , fructose , derivatization , chromatography , reagent , extraction (chemistry) , hydrolysis , gas chromatography–mass spectrometry , gas chromatography , sugar , trimethylsilyl , oxime , mass spectrometry , biochemistry , organic chemistry
Trimethylsilyl (TMS) derivatives of sucrose and TMS oxime derivatives of fructose and glucose were made directly from samples of freeze‐dried fruit tissues without a preliminary extraction of sugars and quantified using gas chromtography. The sugars were identified by a combination of gas chromatography‐mass spectrometry (GC‐MS) and gas chromatographic retention times of authentic compounds. Results were quantitative and reproducible for all samples except tomatoes which had values for sucrose which were significantly more variable than those obtained for fructose and glucose. Hydrolysis of sucrose was minimized and extraction of free sugars was consistent when a 50: 1 ratio of oxime reagent (μL) per mg of dry tissue was used.