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Some Characteristics of the Enzymic Lipid Peroxidation System in the Microsomal Fraction of Flounder Skeletal Muscle
Author(s) -
MCDONALD RICHARD E.,
HULTIN HERBERT O.
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb13964.x
Subject(s) - lipid peroxidation , flounder , chemistry , microsome , biochemistry , singlet oxygen , cofactor , cysteine , enzyme , oxygen , organic chemistry , biology , fish <actinopterygii> , fishery
The microsomal lipid peroxidation system of flounder muscle was characterized as to response to temperature, cofactor requirements, inhibitors, and activators. The enzyme‐catalyzed reaction had a ratelimiting step prior to the formation of lipid hydroperoxides. The K M for NADH of the enzymic system in the presence of 0.1 mM ADP was approximately 1 μM. In the presence of Fe +3 both cysteine and ascorbate also catalyzed lipid peroxidation. While neither NADH nor ADP had an effect in the presence of ascorbate‐Fe +3 , NADH stimulated the cysteine‐Fe +3 oxidation and ADP completely inhibited it. Inhibitor studies indicated that it was a free radical reaction, an ‐SH group was involved and O 2 reduction products and singlet oxygen participated.