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Comparison of Cultural Methods and the DNA Hybridization Test for Detection of Salmonellae in Ground Beef
Author(s) -
EMSWILERROSE B.,
BENNETT B.,
OKREND A.
Publication year - 1987
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1987.tb05914.x
Subject(s) - salmonella , biology , food science , contamination , genetics , bacteria , ecology
A commercially available (Gene‐Trak®) DNA hybridization (DNAH) test was compared with conventional cultural methods for detection of salmonellae in naturally contaminated dairy cow ground beef. All subsamples which were Salmonella‐positive by cultural methods were also positive by DNAH. There was a small percentage (3.7%) of false‐positive subsamples by the DNAH method, but there were no false‐negative results. The DNAH test appears to be a reliable method for detection of salmonellae in ground beef.