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Continuous Hydrolysis of Fructans in Jerusalem Artichoke Extracts using Immobilized Nonviable Cells of Kluyveromyces marxianus
Author(s) -
PAREKH S. R.,
MARGARITIS A.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb13955.x
Subject(s) - jerusalem artichoke , fructan , kluyveromyces marxianus , inulinase , kluyveromyces , fructose , chemistry , hydrolysis , biocatalysis , yeast , bioreactor , chromatography , food science , biochemistry , saccharomyces cerevisiae , organic chemistry , catalysis , ionic liquid
Non‐viable immobilized cells of Kluyveromyces marxianus in alginate beads having inulase (ß‐2,1‐fructan fructano‐hydrolyase E.C.3.2.1.7) acitivity were used as biocatalyst in a packed bed reactor. Extracts of Jerusalem artichoke tubers were contacted with the biocatalyst for continuous conversion of the fructan component to fructose. In a bed reactor packed with 100 mL of beads, a volumetric productivity of 136 g/L/hr total reducing sugars was obtained with 98% substrate conversion. When operated continuously for 30 days, a 55% loss in the original activity was observed, giving a half life for the biocatalyst of 28 days.