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Near Infrared Spectrophotometric Determination of Individual Sugars in Aqueous Mixtures
Author(s) -
GIANGIACOMO ROBERTO,
DULL GERALD G.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb13910.x
Subject(s) - sugar , chemistry , fructose , aqueous solution , sucrose , calibration , spectrophotometry , near infrared spectroscopy , analytical chemistry (journal) , chromatography , food science , mathematics , statistics , optics , organic chemistry , physics
Near infrared region (NIR) spectrophotometry was used to determine concentrations of glucose, fructose and sucrose in aqueous mixtures at 10, 25, and 40% total sugars. Calibration equations and predicted individual concentrations in each total sugar level are reported. The standard errors of prediction varied between 0.35 and 0.69 and confidence limits ranged from ± 0.7 to ± 1.1%. In developing a single equation for predicting individual sugars in solutions, suitable for use over a range of total sugar concentrations, spectral data needed correction for sample density differences. However, at total sugar concentrations above 25%, accompanying wavelength shifts were of sufficient magnitude that a satisfactory single equation could not be developed. Equations for predicting individual sugars in solutions ranging from 10–25% total sugar concentrations were developed. For higher values of total sugars, appropriate calibrations were necessary.