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Optimization of Extracellular Lipase Activity from Pseudomonas fluorescens using a Super‐Simplex Optimization Program
Author(s) -
PAQUETTE G. J.,
McKELLAR R. C.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb13903.x
Subject(s) - pseudomonas fluorescens , lipase , hydrolysis , simplex algorithm , extracellular , chromatography , chemistry , sodium , enzyme , biochemistry , bacteria , biology , organic chemistry , mathematics , linear programming , algorithm , genetics
A method of determining extracellular lipase activity from Pseudomonas fluorescens based on the hydrolysis of β‐naphthyl caprylate has been developed. Optimum activity was determined as a function of temperature, pH, β‐naphthyl caprylate and sodium taurocholate concentrations using a stepwise super‐simplex optimization procedure. Optimization was performed with a Sharp PC‐1500A computer and appropriate ranges for each experimental factor were determined by prior experimentation. A maximum A 540 response of 0.38 units was obtained at 50°C, pH 7.2, 0.1 mM β‐naphthyl caprylate and 17.6 mM sodium taurocholate. This response was 61% better than a previous response obtained by the one‐factor‐at‐a‐time method.