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High Performance Liquid Chromatographic Determination of Vitamin C in Fresh Tomatoes
Author(s) -
RUSSELL L F.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb13864.x
Subject(s) - chromatography , chemistry , vitamin , indophenol , titration , high performance liquid chromatography , vitamin c , food science , biochemistry , inorganic chemistry
A reverse phase ion‐pairing HPLC method for determining vitamin C in fresh tomatoes was developed. An internal standard was used for calibration and quantitation. Recoveries of vitamin C from fresh tomato samples that had been spiked with known amounts of the acid ranged from 96 to 105%. Determination of the vitamin C in fresh tomatoes by this method compared favorably with similar analyses using the standard indophenol titration method.