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Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis
Author(s) -
GORINSTEIN SHELA,
QUICKE GEORGE V.,
PHILLIPS ANNE M.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb13125.x
Subject(s) - polyacrylamide gel electrophoresis , polyacrylamide , chemistry , acetic acid , chromatography , gel electrophoresis , sodium , electrophoresis , sodium dodecyl sulfate , molecular mass , biochemistry , polymer chemistry , organic chemistry , enzyme
Several bands including some low molecular weight (MW) peptides [9 and 11 kd (Kilo‐Dalton)] were visualized in reduced zeins after separation by sodium dodecyl sulphate polyacrylamide gel electro‐phoresis (SDS‐PAGE). It was concluded that 40% isopropanol‐10% acetic acid was effective in fixing these fast moving polypeptides. The demonstration of higher MW (40‐45 Kd) polypeptides may have also resulted from this treatment.