Premium
Staphylococcus aureus Identification Characteristics and Enterotoxigenicity
Author(s) -
BENNETT R.W.,
YETERIAN M.,
SMITH W.,
COLES C.M.,
SASSAMAN M.,
McCLURE F.D.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb13117.x
Subject(s) - lysostaphin , enterotoxin , staphylococcus aureus , coagulase , microbiology and biotechnology , mannitol , agar , biology , micrococcaceae , staphylococcus , chemistry , bacteria , biochemistry , escherichia coli , gene , genetics
Enterotoxigenicity and conventional tests for identification of Staphylococcus aureus were correlated for strains of staphylococci isolated from foods. These strains were examined for enterotoxin production, colonial morphology on Baird‐Parker agar, coagulase activity with rabbit and pig plasma, thermostable nuclease (TNase) production, lysostaphin sensitivity and anaerobic utilization of glucose and mannitol. Enterotoxins A,B,C,D, and E were produced singly or in combination by 100 of the S. aureus strains; 51 strains produced no enterotoxin. False‐negative rates in identifying the enterotoxigenic group as typical S. aureus were as follows: 11% for colonial morphology on Baird‐Parker agar, 8% for coagulase activity with Difco rabbit plasma, 7% for TNase production, 4% for lysostaphin sensitivity and 2% and 4%, respectively, for the anaerobic utilization of glucose and mannitol. Consequently, none of these tests was reliable for differentiating toxigenic from nontoxigenic S. aureus. .