Premium
Quantitative Analysis of Lutein Esters in Marigold Flowers (Tagetes erecta) by High Performance Liquid Chromatography
Author(s) -
GREGORY GAYLE K.,
CHEN TUNGSHAN,
PHILIP THOMAS
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb11248.x
Subject(s) - tagetes , lutein , orange (colour) , carotenoid , chemistry , chromatography , high performance liquid chromatography , solvent , ethyl acetate , botany , organic chemistry , biology , food science
A novel quantitative procedure for the analysis of lutein esters in Marigold flowers (Tageres erecta) using high performance liquid chromatography is described. A new solvent system consisting of methanol and ethyl acetate suitable for the separation of carotenoid hydrocarbons and esters on reversed phase adsorbent was developed for the analysis. Lutein ester concentrations in fresh Marigold flowers varied from 4 pg/g in greenish yellow flowers to 800 pg/g in orange brown flowers. The method is suitable as a general procedure for carotenoid analysis in fruits and flowers where the hydroxylated carotenoids are acylated.