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Enzymatic Conditions of an In Vitro Method to Study Protein Digestion
Author(s) -
GAUTHIER S. F.,
VACHON C.,
SAVOIE L.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb11208.x
Subject(s) - pepsin , proteolysis , digestion (alchemy) , chemistry , casein , enzyme , dialysis , chromatography , hydrolysis , biochemistry , in vitro , peptic , proteolytic enzymes , peptic ulcer , medicine
An in vitro digestion method was proposed for studying protein digestion. Protein (casein) was submitted to peptic proteolysis in a closed system followed by hydrolysis with pancreatic enzymes in a “digestion cell” with continuous elimination of digested products by dialysis. The peptic digestion was performed with a pepsin source of high specific activity (3152 units/mg protein) with an enzyme:substrate (E:S) ratio of 1: 250. The second‐step of proteolysis was carried out with pancreatin for 6 hr at an E:S ratio of 1:25. A 10 mM sodium phosphate buffer, pH 7.5, was used as the circulating dialysis buffer.