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Enzyme‐Linked Immunosorbent Assay for Detection of Mold in Tomato Puree
Author(s) -
LIN H. H.,
LISTER R. M.,
COUSIN M. A.
Publication year - 1986
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1986.tb10865.x
Subject(s) - geotrichum , mold , food science , chemistry , antiserum , enzyme , chromatography , rhizopus , biology , biochemistry , botany , antibody , fermentation , immunology
A double‐sandwich enzyme‐linked immunosorbent assay (ELISA) was developed for the detection of molds ( Ahernaria alternata, Geotrichum candidum and Rhizopus stolonifer ) in tomato puree by the use of antisera raised in rabbits injected with homogenates of the lyophilized boiled molds. Cross reactivity among the three species was less than 10%. Detection limits were approximately 1μg dried mold/g of sample, a sensitivity greater than that of most chemical methods. Positive relationships between ELISA readings and the amounts of mold added to puree were observed, while background ELISA values for puree controls were negligible.