Premium
Spherosil‐S Ion Exchange Process for Preparing Whey Protein Concentrate
Author(s) -
NICHOLS J. A.,
MORR C. V.
Publication year - 1985
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1985.tb13756.x
Subject(s) - chemistry , chromatography , solubility , ion exchange , ultrafiltration (renal) , microporous material , lactose , whey protein , diafiltration , food science , biochemistry , ion , microfiltration , organic chemistry , membrane
Ion exchange whey protein concentrate (IEWPC) was produced from acid whey using Spherosil‐S, a microporous silica bead cation exchange medium. About 80% of the total whey proteins were recovered by the ion exchange process. The composition of IEWPC was 63 ‐ 66% protein, 1 ‐ 2% lactose, 7 ‐ 9% milkfat and 20 ‐ 22% ash. Solubility, PAGE, reverse phase HPLC and Sephadex gel frltration results confirmed that the proteins in IEWPC had undergone substantial denaturation during their isolation. Several major problems encountered during the study were: generation of large volumes of column effluent, the need for concentration of column protein elutate fractions by ultrafiltration, column fouling and loss of protein solubility and functionality.