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Estimation of Microbial Populations in Orange Juice by Bioluminescence
Author(s) -
GRAUMLICH T. R.
Publication year - 1985
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1985.tb13289.x
Subject(s) - bioluminescence , orange juice , orange (colour) , centrifugation , agar , food science , chemistry , microorganism , plate count , chloramphenicol , biology , microbiology and biotechnology , chromatography , bacteria , biochemistry , antibiotics , genetics
Bioluminescent measurement of microbial ATP was compared to plate counts of orange juice on orange serum agar and plate count agar plus chloramphenicol and chlortetracycline. Bioluminescent values correlated well (r = 0.92) with plate counts of reconstituted juices with active microbial populations. A correlation of 0.58 was found for bioluminescent values to plate counts for orange juice samples immediately after reconstitution from concentrate. Centrifugation and resuspension of samples in 0.1M Tris‐HCl buffer, pH 8.0, was utilized to provide adequate pH adjustment and concentration of microorganisms for bioluminescent measurement.