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Effect of Cleaning and Sanitizing on the Attachment of Pseudomonas fragi to Stainless Steel
Author(s) -
STONE LINDA S.,
ZOTTOLA EDMUND A.
Publication year - 1985
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1985.tb12987.x
Subject(s) - scanning electron microscope , chemistry , collagen fibril , fibril , pseudomonas , pseudomonas aeruginosa , atomic force microscopy , microbiology and biotechnology , food science , biophysics , bacteria , biochemistry , materials science , biology , nanotechnology , composite material , genetics
The effects of two clean‐in‐place (CIP) procedures on attachment of Pseudomonas fragi ATCC 4913 to a stainless steel surface were studied using scanning electron microscopy (SEM). Under normal CIP conditions those cells that remained in the system did not exhibit attachment fibrils. Under conditions of lowered detergent water temperature and detergent and sanitizer concentrations, those cells that were present after CIP showed attachment fibrils. Organisms remaining after normal CIP were not viable, whereas those present after suboptimum cleaning were. These viable cells produced attachment fibrils within 24 hr at 21°C and 96 hr at 4°C when grown in milk.