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Simplified Direct Plating Method for Enhanced Recovery of Escherichia coli in Food
Author(s) -
DAMARÉ J. M.,
CAMPBELL D. F.,
JOHNSTON R. W.
Publication year - 1985
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1985.tb10577.x
Subject(s) - escherichia coli , agar , plating (geology) , food science , substrate (aquarium) , chemistry , microbiology and biotechnology , escherichia , fluorescence , food microbiology , chromatography , bacteria , biology , biochemistry , ecology , paleontology , genetics , gene , physics , quantum mechanics
A refined plating medium designed to enumerate E. coli in food within 24 hr was developed. The medium combined tergitol 7 with the fluorogenic substrate, 4‐methylumbelliferyl β‐D‐glucuronide. E. coli colonies were detected by fluorescence under long‐wave UV light. The new agar allowed superior recovery of heat and freeze‐stressed cells and produced E. coli counts from meat and poultry samples at levels generally equivalent to or better than those of the standard 5 tube Most Probable Number method. This method provides a simple and rapid alternative to traditional techniques.