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Effect of Varying Numbers of Isolates from Countable Agar Plates on the Calculated Distribution of Microbial Types in Meats
Author(s) -
VANDERZANT C.,
HANNA M. O.,
SAVELL J. W.
Publication year - 1985
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1985.tb10506.x
Subject(s) - countable set , biology , agar , distribution (mathematics) , veterinary medicine , identification (biology) , plate count , food science , microbiology and biotechnology , mathematics , bacteria , ecology , combinatorics , genetics , medicine , mathematical analysis
Percentage distribution of microbial types in the aerobic microflora of ten meat samples was calculated based on identification of different numbers of isolates (colonies) from countable plates. Numbers of isolates per countable plate used in calculations were (1) all isolates (n), (2) randomly selected isolates (√n, 20, 30, 40 and 50), and (3) one of each of the colony types. With increasing number of randomly selected isolates, differences in distribution of microbial types calculated with a limited number and with all of the isolates became smaller. Microbial distributions calculated on the basis of identification of colony types compared favorably with those calculated on the basis of 30 randomly selected isolates.
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