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Effect of Pectin and Lignin on Mutagenicity in the Ames Assay
Author(s) -
WATSON NANCY Z.,
GREEN NANCY R.
Publication year - 1983
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1983.tb14958.x
Subject(s) - ames test , pectin , lignin , chemistry , pyrene , microsome , salmonella , benzo(a)pyrene , biochemistry , carcinogen , mutagen , food science , bacteria , organic chemistry , biology , in vitro , genetics
ABSTRACT The effect of incorporating varying concentrations of pectin and lignin into the Ames assay with the known mutagens, benzo(a) pyrene, 3‐methyl cholanthrene, and N‐methyl‐N‐nitro‐N‐nitrosoguanidine, on mutagenic activity was investigated. The addition of 50 μg, 150 μg, and 300 μg concentrations of pectin and lignin failed to block the mutagenicity of benzo‐pyrene and 3‐methyl cholanthrene in the Ames/mammalian microsome assay using Salmonella typhimurium TA 98 and TA 100 with and without preincubation. The incorporation of 50 μg, 150 μg, and 300 μg of pectin and lignin did not block the mutagenic activity of MNNG in the Ames Spot test with Salmonella typhimurium TA 100.