Premium
Determination of Tetrodotoxin by Capillary Isotachophoresis
Author(s) -
SHIMADA KAZUKO,
OHTSURU MASARU,
YAMAGUCHI TSUTOMU,
NIGOTA KIMITOSHI
Publication year - 1983
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1983.tb14870.x
Subject(s) - isotachophoresis , tetrodotoxin , chromatography , chemistry , electrolyte , acetic acid , capillary action , potassium , bioassay , biochemistry , biology , biophysics , electrode , materials science , composite material , genetics , organic chemistry
A method is described for the quantification of tetrodotoxin (TTX) in crude extracts of puffer fish by capillary isotachophoresis. Conditions for isotachophoresis consisted of a leading electrolyte of 5 mM potassium acetate, pH 6.0, containing 0.2% Triton X‐100 and 0.5 vol of dioxane, and a terminating electrolyte of 10 mM β‐alanine adjusted to pH 4.5 with acetic acid. It was possible to quantify the TTX content of crude unfractionated puffer extracts, and obtain values similar to those obtained by mouse bioassays which are time consume.