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A New Calculation Method for Distinguishing Endo‐ from Exo‐Polygalacturonases
Author(s) -
TAM STEVEN Y. T.
Publication year - 1983
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1983.tb10782.x
Subject(s) - chemistry , hydrolysis , measure (data warehouse) , chromatography , mathematics , biochemistry , computer science , data mining
A method which uses a comparison ratio (CR) of fluidity (1/n)sp)/μmoles reducing groups is proposed for comparing and distinguishing endo‐ and exo‐polygalacturonases (PGases). This method is faster, easier and as accurate a method as the percent hydrolysis method. The CR method can also be used as a measure of the purity of endo‐ PGase with respect to exo‐PGase.