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High Performance Liquid Chromatographic Determination of Phytic Acid in Foods
Author(s) -
LEE KEN,
ABENDROTH J. A.
Publication year - 1983
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1983.tb09226.x
Subject(s) - phytic acid , chromatography , chemistry , formate , elution , high performance liquid chromatography , linear relationship , biochemistry , catalysis , statistics , mathematics
Phytic acid was quantified using a tetrabutylammonium‐formate ion pair on an octadecyl column in HPLC. Unlike previous methods, phytic acid eluted significantly later than the dead volume. Good separations were possible with a linear response up to 40 micrograms and a sensitivity of 0.002 micrograms phytic acid. Results with this method are compatible with conventional methods for phytic acid.