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Comparison of HPLC and Mouse Bioassay Methods for Determining PSP Toxins in Shellfish
Author(s) -
SULLIVAN JOHN J.,
SIMON MARTHA G.,
IWAOKA WAYNE T.
Publication year - 1983
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1983.tb09218.x
Subject(s) - bioassay , paralytic shellfish poisoning , chromatography , shellfish , high performance liquid chromatography , toxin , toxicity , shellfish poisoning , chemistry , detection limit , biology , fishery , fish <actinopterygii> , aquatic animal , biochemistry , ecology , organic chemistry
Paralytic shellfish poisoning (PSP) toxins in shellfish were analyzed and quantified using both a high pressure liquid chromatographic (HPLC) technique and the standard AOAC mouse bioassay. Good correlation between the two assays was obtained at or below the limit of 80 μg toxin/100g, while at higher levels of toxicity, the HPLC technique tended to underestimate total toxicity slightly. Advantages and disadvantages of both techniques are discussed.