Premium
Radiation‐Injured CIostridium Botulinurn Type E Spores: OutGrowth and Repair
Author(s) -
ROWLEY DURWOOD B.,
FIRSTENBERGEDEN RUTH,
SHATTUCK G. EDGAR
Publication year - 1983
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1983.tb05095.x
Subject(s) - spore , neomycin , microbiology and biotechnology , agar , polymyxin , germination , lysis , polymyxin b , chemistry , irradiation , lytic cycle , biology , antibiotics , bacteria , botany , immunology , biochemistry , genetics , physics , nuclear physics , virus
Spores of Clostridium botulinum type E surviving irradiation doses of 0.1–0.4 Mrad were injured as evidenced by their inability to form typical macrocolonies at 10°C in the presence of polymyxin and neomycin, antibiotics which did not similarly affect growth of unirradiated spores. Several degrees of severity of injury were demonstrated. Radiation injury was not associated with the germination lytic system but with the post germination growth system. Radiation‐injured spores formed aseptate filaments during outgrowth in microcultures. Some of these filaments lysed; others divided and formed microcolonies less dense than those formed by unirradiated cells. Radiation‐injured spores repaired when incubated at 30°C for ca. 15 hr on TP‐EY agar medium with polymyxin and neomycin, and formed typical macrocolonies when shifted to 10°C.