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A Rapid Gas Chromatographic Method for the Identification of Sporeformers and Nonsporeformers in Swollen Cans of Low‐Acid Foods
Author(s) -
SCHAFER M. L.,
PEELER J. T.,
BRADSHAW J. G.,
HAMILTON C. H.,
CARVER R. B.
Publication year - 1982
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1982.tb12939.x
Subject(s) - chromatography , chemistry , gas chromatography , flame ionization detector , food science
A rapid gas‐liquid chromatographic method for the identification of sporeformers and nonsporeformers as the probable cause of swollen cans of low‐acid foods was developed and evaluated. Cans of five types of food were inoculated with cultures of seven sporeforming organisms and three nonsporeformers. The supernatant from the can content was assayed on two packed columns designed for the assay of aqueous solutions of volatile free acids using flame ionization detectors. A statistical discriminant analysis was conducted on selected peak areas. Overall, 88% of 120 cans were correctly identified in the standard data set and 80% of 110 cans analyzed as unknowns. Sporeformers were detected in 62% of 30 cans inoculated with three mixtures of sporeformers and nonsporeformers.