Premium
Comparison of Selected Yersinia enterocolitica Indicator Tests for Potential Virulence
Author(s) -
STERN NORMAN J.,
DAMARÉ JOHN M.
Publication year - 1982
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1982.tb10128.x
Subject(s) - virulence , yersinia enterocolitica , plasmid , microbiology and biotechnology , biology , pathogenicity , bacteria , genetics , gene
Three recently developed tests used to indicate virulence were performed on 34 strains of Yersinia enterocolitica. Agreement in virulence or nonvirulence prediction was found in (a) 29 of 31 strains when tested for calcium growth dependency at 35°C; (b) all 34 strains when tested for the ability to autoagglutinate in tissue culture medium at 35°C; and (c) among all 34 strains when tested for the presence of a 40–48 Mdal plasmid using a modification of the Hansen and Olsen method for plasmid analysis. The autoagglutination assay is recommended for testing potential pathogenicity of food‐borne isolates. There are probably several virulence‐determining factors for Y. enterocolitica , and no one in‐vitro test will suffice. The analytical procedure described herein can be used to detect plasmids ranging in size from approximately 2 × 10 6 to 3 × 10 8 daltons.