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Ultrastructural Analysis of a Soybean Protein Isolate
Author(s) -
DIECKERT JULIUS W.,
DIECKERT MARILYNE C.
Publication year - 1982
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1982.tb07669.x
Subject(s) - ultrastructure , transmission electron microscopy , fixation (population genetics) , food science , moisture , chemistry , biology , biochemistry , materials science , botany , composite material , nanotechnology , gene
Various fixation techniques were employed in an effort to prepare a dry protein isolate from soybeans for analysis using transmission electron microscopy. A number of fixation schemes proved successful, and different properties of the material were brought out by the various treatments. Comparisons of electron micrographs of the isolate fixed and extracted in different ways yielded an effective means for determining the distribution of fat and protein in the sample and the effect of moisture on that distribution. Analysis of the isolate demonstrated the occluding effect of dry protein for fat located in walls of the protein shells of the isolate.