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A Method for the Analysis of ATP Metabolites in Beef Skeletal Muscle by HPLC
Author(s) -
CURRIE RONALD W.,
SPORNS PETER,
WOLFE FRED H.
Publication year - 1982
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1982.tb07653.x
Subject(s) - hypoxanthine , inosine , chromatography , chemistry , high performance liquid chromatography , homogenization (climate) , nad+ kinase , skeletal muscle , metabolite , biochemistry , enzyme , anatomy , biology , biodiversity , ecology
A method to determine inosine, hypoxanthine, NAD, AMP, IMP, IDP, ADP, adenylosuccinic acid, ITP and ATP contents in postmortem muscle by HPLC has been developed. The method consists of an homogenization of the muscle in 0.5M HClO 4 or 10% TCA, extraction of the compound with 0.5M tri‐n‐octylamine/Freon 113 and single injection chromatography on a Whatman Partisil‐10/25 SAX column.