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HPLC Determination of Fructose, Glucose, and Sucrose in Potatoes
Author(s) -
WILSON A. M.,
WORK T. M.,
BUSHWAY A. A.,
BUSHWAY R. J.
Publication year - 1981
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1981.tb14589.x
Subject(s) - fructose , sucrose , chromatography , chemistry , high performance liquid chromatography , acetonitrile , carbohydrate , solvent , sugar , food science , biochemistry
ABSTRACT A high‐performance liquid chromatographic (HPLC) method has been developed to determine fructose, glucose, and sucrose content of potatoes. The HPLC system consisted of a μBondapak/ carbohydrate column, a solvent system of acetonitrile‐water (75: 25), a flowrate of 1.8 ml/min, and a refractive index detector. Analysis, including sample preparation, was complete in 30 min. With the exception of high concentrations of sucrose (8 mg/g or above), the method recovered 93% or more of all sugars. The coefficients of variation for the procedure ranged from 1.39‐13.31%. TLC indicated that there were no interfering compounds eluting with any of the three sugars.