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Recovery of Salmonellae from Foods using a Combined Enrichment Technique
Author(s) -
SVEUM W.H.,
KRAFT A.A.
Publication year - 1981
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1981.tb14538.x
Subject(s) - tetrathionate , selenium , lactose , cystine , incubation , chemistry , food science , salmonella , chromatography , biology , biochemistry , bacteria , genetics , organic chemistry , cysteine , enzyme
The separate steps of preenrichment in lactose broth and selective enrichment in tetrathionate (TET) or selenite‐cystine (SC) broths were combined into single procedures for the detection of both injured and uninjured salmonellae in foods. Following 4 hr of incubation, iodine or selenite were added to combined lactose‐tetrathionate (CTET) or selenite basal broths, forming selective environments. CTET broth was as efficient as conventional TET and SC broths but the combined selenite media were not. Salmonellae were detected in foods in 30 hr using direct fluorescent antibody and combined enrichment techniques. The combined procedure resulted in savings of assay time, labor and materials.