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Effect of Reaction Conditions on Hydrolysis of Chitin by Serratia marcescens QMB 1466 chitinase
Author(s) -
TOM RAMOND A.,
CARROAD PAUL A.
Publication year - 1981
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1981.tb04934.x
Subject(s) - serratia marcescens , chitin , chitinase , hydrolysis , bioconversion , chemistry , laboratory flask , erlenmeyer flask , enzymatic hydrolysis , chromatography , enzyme , nuclear chemistry , chemical engineering , organic chemistry , chitosan , biochemistry , escherichia coli , fermentation , gene , engineering
The hydrolysis of chitin by Serratia marcescens chitinase was studied as part of ar overall project to develop a chitin waste treatment bioconversion scheme. Hydrolyses were conducted with automatic pH control in stirred flasks. Parameters varied were temperature, enzyme activity, and chitin particle size and concentration. A temperature of 30°C is suitable. Hydrolysis increases with increasing enzyme activity and chitin slurry concentration, and with decreasing particle size.