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Analysis of Dietary Fiber in Feces of Rats Fed with Fiber Supplemented Diets
Author(s) -
RASPER V. F.,
BRILLOUET J. M.,
BERTRAND D.,
MERCIER C.
Publication year - 1981
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1981.tb04910.x
Subject(s) - fractionation , chemistry , bran , chromatography , hydrolysis , fiber , cellulose , food science , feces , uronic acid , digestion (alchemy) , carbohydrate , polysaccharide , biochemistry , biology , organic chemistry , raw material , microbiology and biotechnology
A procedure based on Southgate's fractionation scheme with subsequent colorimetric determination of monomeric constituents in the individual fractions was used for the analysis of feces of rats fed with diets supplemented with cereal bran or its fiber components. Prior to fractionation, dehydrated feces were extracted with chloro‐form:methanol (2:1) mixture and hot 80% methanol and then subjected to treatment with both amylolytic (MERCK glucoamylase, 60°C, 2 hr) and proteolytic (CALBIOCHEM pepsin, 40°C, 48 hr) enzymes. Colorimetrically determined values of noncellulosic polysaccharides and cellulose (calculated as sums of hexoses, pentoses, and uronic acids) agreed satisfactorily with those determined by detergent fiber analysis. The sums of neutral sugars determined colorimetrically correlated closely with the values obtained by GLC analysis after Saeman's hydrolysis (2 hr with 72% (w/w) H 2 SO 4 at 20°C and 2 hr with 2N H 2 SO 4 at 100°C) of defatted samples. The GLC data were, however, consistently higher, especially those for hexoses; the difference was more pronounced with feces than with tested bran material. Confidence in the analysis was strengthened by the observation that the results of proximate and fiber analyses closely approached 100%.