Premium
Discrimination of Bacillus cereus from Selected Bacillus spp. by Pyrolysis Gas‐Liquid Chromatography
Author(s) -
STERN NORMAN J.
Publication year - 1981
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1981.tb04190.x
Subject(s) - bacillus cereus , bacilli , linear discriminant analysis , biology , gas chromatography , cereus , bacillus (shape) , chromatography , chemistry , microbiology and biotechnology , bacteria , mathematics , statistics , genetics
Pyrolysis gas‐liquid chromatography (PGLC) and statistical analysis employing stepwise discriminant analysis (SDA), were used to classify five species and varieties in the genus Bacillus. Nine strains were harvested, lyophilized, and pyrolyzed under replicate conditions. The combination of PGLC and SDA enabled classification of these selected varieties and species of Bacillus at 96% accuracy. Stepwise discriminant analysis of the same PGLC data discriminated (100%) B. cereus , a foodborne pathogen, from the remaining selected nonpathogenic bacilli when the two groups were compared. One particular elution peak resulting from the PGLC‐SDA analysis was prominent in both discriminations reported. The PGLC and SDA method offers potential as an accurate, objective procedure for the discrimination of these selected bacilli.