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Production of Thermonuclease Deficient Mutants from an Enterotoxigenic Thermonuclease Producing Strain of Staphylococcus aureus
Author(s) -
BATISH V. K.,
CHANDER H.,
GHODEKAR D. R.,
RANGANATHAN B.
Publication year - 1981
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1981.tb03033.x
Subject(s) - enterotoxin , staphylococcus aureus , microbiology and biotechnology , reversion , strain (injury) , mutant , acriflavine , biology , lecithinase , phenotype , bacteria , biochemistry , gene , escherichia coli , genetics , anatomy
A toxigenic strain of Staphylococcus aureus known to produce both thermonuclease and enterotoxin was exposed to ultraviolet radiations. Thermonuclease deficient mutants were recovered on methylgreen‐DNA‐agar medium. All of twenty thermonuclease deficient mutants lost the capacity to produce coagulase, enterotoxin, and lecithinase. However, these functions were regained upon reversion with visible light (Photoreactivation) thus suggesting a coordinated control mechanism for the synthesis and release of the above metabolites. These characteristics in most of the mutants remained stable for 3 months.