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CAULIFLOWER LYSOZYME
Author(s) -
EREIFEJ K. I.,
MARKAKIS P.
Publication year - 1980
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1980.tb07612.x
Subject(s) - lysozyme , lysis , enzyme , chemistry , polyacrylamide gel electrophoresis , electrophoresis , chromatography , micrococcus , gel electrophoresis , polyacrylamide , biochemistry , biology , bacteria , polymer chemistry , genetics
An enzyme capable of lysing the cell walls of Micrococcus lysodeicticus was isolated from cauliflower. In SDS polyacrylamide gel electrophoresis at pH 7.2, this enzyme behaved like a basic polypeptide with 21,700 MW. The pH optimum for activity was 6.0, the K m was 0.33 mg dry cell walls per ml, and the V max was 0.2 A 5 4 0 units per min.