Premium
AVOCADO CELLULASE EXTRACTION AND PURIFICATION
Author(s) -
AWAD MARCEL,
LEWIS LOWELL N.
Publication year - 1980
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1980.tb07577.x
Subject(s) - cellulase , isoelectric point , chromatography , chemistry , extraction (chemistry) , isoelectric focusing , cellulose , enzyme , electrophoresis , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry
Optimum conditions for the extraction and purification of avocado cellulase have been established. The best quantiative recovery of the cellulase from avocado tissue was obtained with a 0.04M acetate buffer, pH 5.0, containing 0.4M NaCl and 0.25% (v/v) Triton X‐100. The isoelectric point of the enzyme was 4.7 and the molecular weight, based on SDS gel electrophoresis, was 49,000. The enzyme was purified on a cellulose column to a single protein band on SDS gel electrophoresis. Rabbit antibody produced to the cellulase gave further proof of the enzyme's purity.