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THIAMIN AND RIBOFLAVIN ANALYSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
KAMMAN J. F.,
LABUZA T. P.,
WARTHESEN J. J.
Publication year - 1980
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1980.tb07548.x
Subject(s) - riboflavin , high performance liquid chromatography , chromatography , chemistry , absorbance , elution , food science
A technique utilizing high‐performance liquid chromatography (HPLC) was developed for the analysis of thiamin and riboflavin in enriched and fortified foods. The vitamins were extracted from cereals and processed cereal products and simultaneously assayed by reverse‐phase HPLC. The vitamins eluted as separate peaks within 8 min and were detected by absorbance at 254 nm. The developed technique was compared to the semi‐automatic modification of the AOAC method for seven cereal products. There was no statistical difference found in the values obtained from the two procedures.