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ANALYSIS OF POTATO GLYCOALKALOIDS BY HIGH‐PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
BUSHWAY R. J.,
BARDEN E. S.,
WILSON A. M.,
BUSHWAY A. A.
Publication year - 1980
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1980.tb07528.x
Subject(s) - high performance liquid chromatography , chemistry , chromatography , glycoalkaloid , coefficient of variation , titration , solanaceae , biochemistry , inorganic chemistry , gene
A high‐performance liquid chromatographic (HPLC) procedure to quantitatively determine α‐chaconine and α‐solanine in potato tubers was developed. Five varieties of tubers, stored at two different temperatures for 9 months, were analyzed for their α‐chaconine and α‐solanine content. The values for α‐chaconine ranged from 3.89–23.04 mg/100g fresh tuber whereas for α‐solanine the amount varied from 1.69–12.51 mg/100g fresh tuber depending on the variety. The coefficient of variation was rather high between different samples of the same tuber variety, but for the same samples, the coefficient of variation was excellent (1.6–6.3%). The amount of glycoalkaloids determined by HPLC is in good agreement with those values obtained from the widely used titration method.