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COMPARISON OF METHODS FOR SEPARATING OLIGOSACCHARIDES: ULTRAFILTRATION, GEL PERMEATION AND ADSORPTION CHROMATOGRAPHY
Author(s) -
WANISKA R. D.,
KINSELLA J. E.
Publication year - 1980
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1980.tb06533.x
Subject(s) - chemistry , chromatography , ultrafiltration (renal) , gel permeation chromatography , elution , size exclusion chromatography , adsorption , membrane , permeation , organic chemistry , biochemistry , polymer , enzyme
Methods for the separation of glucose oligomers with a degree of polymerization (DP) of 5‐10 and l0‐20 were studied. Gel filtration using Bio‐Gel P‐2 fractionated glucose oligomers from corn syrups with dextrose equivalents (DE) of 36, 43, and 62. Corn syrup DE 43 was fractionated with Diaflow ultrafiltration (UF) membranes PMl0, UMl0, and UM2. Adsorption chromatography of corn syrup DE 43 was conducted with a charcoal‐celite column using a step‐wise elution schedule of ethanol:water mixtures. Gel permeation chromatography provided elution profiles of the various corn syrups; however, the respective glucose oligomers were not clearly resolved. Corn syrup DE 43 had the most oligosaccharides with the least content of sugars of large or small molecular size. Ultrafiltration using the UM2 and UMl0 membranes retained from 55‐70s of the oligosaccharides. Less than 7% of the oligomers were retained by the PM10 membrane. Adsorption chromatography was most efficient and effective in the separation of the discrete fractions with DP of 5‐10 and 10‐20.