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A LIQUID SCINTILLATION TECHNIQUE FOR MICROBIAL VITAMIN ANALYSES
Author(s) -
VOIGT M. N.,
EITENMILLER R. R.
Publication year - 1979
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1979.tb09140.x
Subject(s) - pantothenic acid , pyridoxine , yeast , absorbance , chemistry , liquid scintillation counting , vitamin , bacteria , chromatography , food science , euglena , thiamine , biochemistry , biology , chloroplast , gene , genetics
A radiometric procedure for the assay of the B‐vitamins was investigated. The method, based on the release of 14 CO 2 from C‐14 labeled glucose, gave proportional responses to vitamin concentration for thiamin, pyridoxine, and pantothenic acid. This procedure was not significantly faster than measuring increases in absorbance due to growth when bacteria and yeast were used as test organisms. However, for the protozoan assay of thiamin with Ochromonas danica , the analysis time was decreased from 4‐5 days to 20 hours.