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AN IMPROVED SEMIAUTOMATED ENZYMATIC ASSAY OF LYSINE IFU FOODSTUFFS
Author(s) -
ROY R. B.
Publication year - 1979
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1979.tb03816.x
Subject(s) - lysine , lysine decarboxylase , chemistry , chromatography , phenolphthalein , enzyme , amino acid , reproducibility , metal ions in aqueous solution , carbon dioxide , biochemistry , ion , organic chemistry , putrescine , cadaverine
An improved semiautomated enzymatic procedure employing L‐ly‐sine decarboxylase for the analysis of lysine is described. Carbon dioxide formed in the reaction mixture is separated by a gas dialyzel and measured calorimetrically by the phenolphthalein method. Certain vitamins, amino acids, and metal ions, when added to lysine standard solution and analyzed by the present procedure, caused a decrease in both sensitivity and reproducibility. The procedure developed is capable of analyzing 30 samples per hour. The mean recovery of added lysine was 94.1%.