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LECTINS FROM RED KIDNEY BEANS: RADIATION EFFECT ON AGGLUTINATING AND MITOGENIC ACTIVITY
Author(s) -
FILHO J. MANCINI,
LAJOLO F. M.,
VIZEU O. M.
Publication year - 1979
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1979.tb03479.x
Subject(s) - hemagglutinin (influenza) , ionizing radiation , chemistry , kidney , polyacrylamide gel electrophoresis , lectin , immunoelectrophoresis , biochemistry , microbiology and biotechnology , biology , immunology , irradiation , antibody , endocrinology , physics , nuclear physics , gene , enzyme
The authors studied the effect of ionizing radiations on hemagglutinating and mitogenic activity of purified phytohemagglutinin in solution. A dose of 10 Krad, is needed for 50% destruction of the agglutinating capacity; the mitogenic effect is more resistant: 70 Krad for 50% inactivation. The results indicate that the agglutinating and mitogenic effects are due to different structures. The radiation effect on agglutinating activity is an exponential function of the lectin concentration. Macromolecular changes, followed by polyacrylamide gel electrophoresis, were evident for doses high than 40 Krad. A dose up to 3 Mrad was ineffective to inactivate the hemagglutinin in kidney beans even with 30% or 60% water content.