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GROWTH OF Bifidobacterium bifidum VAR. Pennsylvanicus IN LABORATORY MEDIA SUPPLEMENTED WITH AMINO SUGARS AND SPENT BROTH FROM Escherichia coli
Author(s) -
JAO Y. C.,
MIKOLAJCIK E. M.,
HANSEN P. M. T.
Publication year - 1978
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1978.tb15282.x
Subject(s) - bifidobacterium bifidum , food science , actinomycetaceae , bifidobacterium , escherichia coli , microbiology and biotechnology , chemistry , biology , lactobacillus , biochemistry , fermentation , gene
Growth stimulation of B. bifidum var. pennsylvanicus by filter‐ sterilized, unheated amino sugars was studied utilizing an ATCC medium 76 assay system. The order of stimulatory effect was N‐acetylglu‐ cosamine (GlcNAc) > N‐acetylgalactosamine > N‐acetylmannosamine > N‐acetylneuraminic acid. When GlcNAc was heated at 121°C for 15, 30 and 45 min, depression of growth occurred and was directly related to the heat treatment applied. The presence of E. coli B organisms in the assay system did not influence the growth stimulatory effect of the amino sugars on B. bifidum Spent broth, prepared by growth of E. coli B in the assay medium followed by filter sterilization, was found to stimulate the subsequent growth of B. bifidum . The spent broth contained small quantities of amino sugars. E. coli B failed to grow in spent broth prepared from B. bifidum without pH adjustment. Growth of E. coli B was observed when this spent medium was neutralized and fortified with amino sugars, however, without any specific stimulatory pattern attributable to the type of amino sugar.