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IDENTIFICATION OF STARCH AND PROTEIN COMPONENTS OF GREEN BANANA TISSUE BY SCANNING ELECTRON MICROSCOPY AND USING SELECTIVE STAINS FOR X‐RAY FLUORESCENT MICROANALYSIS
Author(s) -
MEDINA MARJORIE B.,
GREENHUT VICTOR A.,
LACHANCE PAUL A.
Publication year - 1978
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1978.tb09748.x
Subject(s) - starch , fluorescence , scanning electron microscope , electron microscope , microanalysis , chemistry , nuclear chemistry , biochemistry , materials science , organic chemistry , optics , physics , composite material
Starch and protein components of green banana tissue ( Musa cavendish ) were selectively complexed with iodine and copper respectively. The tissue was characterized with a scanning electron microscope by employing X‐ray fluorescent emission (EDS) to locate starch and protein components by their relevant stain and secondary emission. Starch granules in the tissue gave strong iodine signals and revealed a structure similar to isolated granules. Electron‐opaque substances surrounding the starch granules provided a strong copper signal indicating its protein‐aceous nature. Other elements used in tissue preparation revealed no specificity to the tissue components. It is apparent that specific tissue components can be identified by selective labeling with elements for electron‐microbeam X‐ray fluorescent analysis.