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PURIFICATION OF PORCINE LEUKOCYTE LYSOSOMAL HYDROLASES
Author(s) -
VENUGOPAL BALAKRISHNA,
BAILEY MILTON E.
Publication year - 1978
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1978.tb07405.x
Subject(s) - sephadex , acid phosphatase , cathepsin d , chemistry , chromatography , polyacrylamide gel electrophoresis , cathepsin , differential centrifugation , centrifugation , biochemistry , column chromatography , electrophoresis , enzyme
Leukocytes were separated from porcine blood and their cellular components fractionated either by differential centrifugation or by n‐butyl alcohol extraction. Acid phosphatase, aryl sulfatases, β‐glucuronidase and cathepsin D were purified by DEAE‐cellulose and Sephadex column chromatography and by polyacrylamide gel electrophoresis. Histochemical reactions were used to identify enzymes in the latter procedure.