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POSTMORTEM AGING OF BOVINE MUSCLE: A COMPARISON OF TWO PREPARATION TECHNIQUES FOR ELECTRON MICROSCOPY
Author(s) -
VARRIANOMARSTON E.,
DAVIS EUGENIA A.,
HUTCHINSON T. E.,
GORDON JOAN
Publication year - 1978
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1978.tb02392.x
Subject(s) - ultrastructure , myofibril , electron microscope , transmission electron microscopy , postmortem changes , microscopy , chemistry , biophysics , anatomy , pathology , materials science , biology , medicine , biochemistry , nanotechnology , optics , physics
Two preparatory techniques for electron microscopy were employed to investigate ultrastructural changes in bovine muscle during postmortem aging. The ultrastructural changes in aged muscle prepared by ultrarapid frozen thin sectioning procedures were less extensive than those observed in fixed, dehydrated, and embedded muscle, and this suggested that the preparation procedure may affect observed structural characteristics. The major changes observed in unfixed, frozen hydrated muscle were evident as alterations in electron scattering in the striations of the myofibrils indicating that ion redistribution occurred during postmortem aging. Structural alterations observed in aged tissue prepared by standard techniques included disruptions in the Z line, breaks at the Z‐I junction and fading or disappearance of the M line.