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DISTRIBUTION OF BEAN AND WHEAT INOSITOL PHOSPHATE ESTERS DURING AUTOLYSIS AND GERMINATION
Author(s) -
FERREL ROBERT E.
Publication year - 1978
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1978.tb02355.x
Subject(s) - autolysis (biology) , phytic acid , phosphate , chemistry , germination , hydrolysis , inositol , phytase , inositol phosphate , food science , biochemistry , botany , biology , enzyme , receptor
Ion‐exchange chromatography was used to follow changes in inositol phosphate esters of California small white beans and hard red winter wheat during autolysis and germination. Untreated bean extracts contained appreciable quantities of lower phosphate esters, while wheat extracts contained only inositol hexaphosphate (IHP). Autolysis in beans at both 35 and 55°C was slow (30% of IHP still present after 48 hr). Over 80% of wheat IHP was hydrolyzed in 4 hr at both temperatures. During autolysis bean extracts contained appreciable quantities of lower phosphate esters while wheat chromatograms showed trace amounts. Germination of the seeds for 2, 4 and 7 days produced similar patterns of change in the phosphate esters as in autolysis. The use of wheat phytase to rapidly reduce phytic acid in bean preparations is presented.