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TWENTY‐FOUR HOUR METHODS FOR BACTERIOLOGICAL ANALYSES IN FROZEN RAW BREADED SHRIMP PROCESSING
Author(s) -
GODWIN GLYN J.,
GRODNER ROBERT M.,
NOVAK ARTHUR F.
Publication year - 1977
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1977.tb12595.x
Subject(s) - shrimp , coagulase , food science , staphylococcus aureus , plate count , significant difference , agar , veterinary medicine , mathematics , biology , staphylococcus , statistics , fishery , medicine , bacteria , genetics
24‐hr procedures suggested for the bacteriological evaluation of critical control points in the processing of frozen raw breaded shrimp were compared to official techniques on an interlaboratory basis. The 24‐hr methods were designed to enumerate APC, total coliforms, E. coli , and coagulase‐positive Staphylococcus aureus . The 24‐hr APC was a standard plate count procedure using Eugon agar, while the abbreviated techniques for total coliforms, E. coli , and coagulase‐positive S. aureus were 3‐tube MPN procedures. The data gathered from 16 analytical laboratories comprising 18 series of frozen raw breaded shrimp subsamples were subjected to a least mean square analysis to determine the effects of technique, the bivariate interaction technique x laboratory, and the variable “SM” which embodied the sample source and month processed. The analyses of variance indicated that there were no differences between techniques for APC, total coliforms, and E : coli at the 5% level of significance. The bivariate interaction, technique x laboratory, was not significant at the 5% level for these tests indicating a consistent relationship between the means for the 24‐hr and official methods for the various tests. The difference between the 24‐hr and official method for coagulase‐positive S. aureus was significant at the 10% level and the interaction, technique x laboratory, was highly significant. The variable SM was highly significant for all tests.