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RAPID IDENTIFICATION OF Salmonella AND RELATED FOODBORNE BACTERIA BY FIVE BIOCHEMICAL MULTITEST SYSTEMS
Author(s) -
POELMA P. L.,
ROMERO A.,
ANDREWS W. H.
Publication year - 1977
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1977.tb12577.x
Subject(s) - salmonella , microbiology and biotechnology , enterobacteriaceae , identification (biology) , biology , bacteria , enteric bacteria , food science , escherichia coli , biochemistry , botany , genetics , gene
An evaluation was made of five commercially available “multitest systems” suggested for biochemically differentiating members of the Enterobacteriaceae . Three biochemical kits (Enterotube, PathoTec Rapid I‐D System, and Modified Expanded R/B Enteric Differential System) gave incorrect identification for two of 110 Salmonella cultures, whereas two kits (API‐20 Enteric and Minitek Differential System) gave correct identification of all 110 Salmonella cultures. The PathoTec Raoid I‐D Svstem gave incorrect identification for two of 15 non‐ Salmonella , whereas the remaining four kits gave incorrect identification for three of these cultures. The overall average correlation for all biochemical tests or substrates evaluated within each system was as follows: API, 99%; Enterotube, 99%; Minitek, 99%; PathoTec, 97%; and R/B, 96%.