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RECOVERY OF BACTERIAL ENDOSPORES FROM A METAL SURFACE AFTER TREATMENT WITH HYDROGEN PEROXIDE
Author(s) -
NEAL N. D.,
WALKER H. W.
Publication year - 1977
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.1977.tb08436.x
Subject(s) - bacillus cereus , spore , hydrogen peroxide , clostridium sporogenes , cereus , incubation , chemistry , endospore , agar , microbiology and biotechnology , starch , food science , nuclear chemistry , bacteria , clostridium , biochemistry , biology , genetics
Spores of Bacillus cereus NRRL 3476 and of Clostridium sporogenes ATCC 7995 (PA 3679) were inoculated onto stainless steel surfaces. After the spores had dried on the surface, the surface and spores were exposed to 9.2% hydrogen peroxide for sufficient time to achieve a two‐log reduction in the number of viable cells. The number of surviving spores was determined by pouring the agar media directly onto the stainless steel surface and incubating for appropriate lengths of time. When spores of B. cereus or PA 3679 were treated with hydrogen peroxide, their recovery was enhanced if the pH of the recovery medium was adjusted to pH 7.3. Further increase in recovery occurred if 0.5% soluble starch was added to the recovery medium for B. cereus and if 5 μM of glutathione/ml was added to the recovery medium of PA 3679. Temperatures of incubation in the range of 28‐36°C had no influence on recovery.